Explain the various steps involved in the production of artificial insulin cbse Biology class 12

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Explain the various steps involved in the production of artificial insulin. cbse-Biology-class-12

Explain the various steps involved in the production of artificial insulin.


By:tarun101

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Steps involved in production of artificial insulin:

a) Using specific techniques, the plasmid of E. coli is removed and isolated.
b) The isolated plasmid is opened by specified enzyme.
c) The segment of DNA which codes for human insulin is inserted in the open site of the bacterial plasmid.
d) After  insertion,  the  plasmid  is  closed  by  using  another  special  enzyme  to make it a recombinant plasmid.
e) This recombinant plasmid is again introduced into E. coli host cells.
f) Now, E.  coli cells become  capable  of  synthesising  human  insulin.  These bacteria  are  then  cultivated  in  fermenters  to  produce a large  amount  of insulin.
g) Insulin is then extracted from bacterial culture and purified.

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