Name and describe the two types of partition chromatography cbse biotechnology class11 2014

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Name and describe the two types of partition chromatography. cbse-biotechnology-class11-2014

Name and describe the two types of partition chromatography. 


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Adsorption chromatography contains an absorbent as a stationary phase whereas affinity chromatography is based on a highly specific biologic interaction. The column chromatography contains a column in which various solutes of a solution are travelled and separate individual components on the stationary phase. 
The column chromatography is used to separate biomolecules like vitamins, steroids, hormones etc.  It also determines the amounts of constituents of mixture. In the exclusion chromatography, the stationary phase is a gel with closely controlled pore size. In this method, molecules are separated on the basis of molecular size and shape. 
Gas chromatography is used an inert gas as the mobile phase to separate the components of a mixture. Paper chromatography contains a sheet of blotting paper or filter paper in the adsorption column. The partition chromatography is a process of separation that utilizes the partition of the solutes between two liquid phases.


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